Part:BBa_J119439:Design

BCL-2 Promoter G-quadruplex and i-Motif

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

I had to place 5' aatt 3' sticky ends on either end of the sequence so it could be ligated into a plasmid digested with Eco RI. When it is ligated, it can have either its template strand or its coding strand 5' to 3'.

Source

This part is from the human genome. The BCL-2 oncogene promoter contains this i-motif/G-Quadruplex-forming sequence.